Figure 6. Depletion of hepatic Surf4 by siRNA recapitulates the hypolipidemia seen in Surf4^fl/fl Alb-Cre⁺ mice.

(A) Normalized (Norm.) liver Surf4 mRNA levels in mice treated with scrambled siRNA (control) or varying concentrations of Surf4 targeting siRNA (n=5 per group). (B–D) Plasma PCSK9, cholesterol, and triglyceride levels in control and siRNA treated mice. (E–F) Plasma levels of asparate aminotransferase (AST) and alanine transaminase (ALT) in mice treated with control or increasing doses of Surf4 targeting siRNA. Statistical significance was computed by one-way ANOVA test followed by Tukey’s post hoc test.

Figure 6—figure supplement 1. Immunoblots of plasma and liver lysates collected from mice treated with control or increasing doses of Surf4 targeting siRNA (Figure 6—figure supplement 1—source data 1).