Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Ambystoma mexicanum) | Axolotl, white (d/d) | CRTD Axolotl facility | ||
| Genetic reagent (A. mexicanum) | Sox9:Sox9-T2a-mCherrynls | Riquelme-Guzmán et al., 2021 | C-Tit/+(Sox9:Sox9-T2a-mCherrynls)ETNKA | |
| Genetic reagent (A. mexicanum) | Ctsk:mRuby3 | This paper | tgTol2(Drer.Ctsk:mRuby3)tsg | Generated in d/d strain |
| Genetic reagent (A. mexicanum) | Ctsk:eGFP | This paper | tgTol2(Drer.Ctsk:eGFP)tsg | Generated in d/d strain |
| Genetic reagent (A. mexicanum) | Sox9 × Ctsk | This paper | C-Tit/+(Sox9:Sox9-T2a-mCherrynls)ETNKA × tgTol2(Drer.Ctsk:eGFP)tsg |
|
| Antibody | Rabbit polyclonal anti-CTSK | Abcam | Cat #ab1902 RRID:AB_2261274 |
Immunofluorescence (IF; 1:20) |
| Antibody | Rat monoclonal anti-F4/80 | Biorad | Cat #MCA497 RRID:AB_2335599 |
IF (1:100) |
| Antibody | Sheep polyclonal anti-digoxigenin-AP, Fab fragments | Roche | Cat #11093274910 RRID:AB_2313640 |
In situ hybridization (ISH; 1:5000) Whole mount ISH (1:3000) |
| Recombinant DNA reagent | p-GEMt-Ctsk | This paper | ISH probe | |
| Recombinant DNA reagent | p-GEMt-Kazald1 | This paper | ISH probe | |
| Recombinant DNA reagent | p-GEMt-Krt17 | This paper | ISH probe | |
| Recombinant DNA reagent | Ctsk:mRuby3 | Backbone from Geurtzen et al., 2022 | Modified for this paper | |
| Recombinant DNA reagent | Ctsk:eGFP | Backbone from Geurtzen et al., 2022 | Modified for this paper | |
| Sequence-based reagent | Ctsk Fw primer | Bryant et al., 2017 | PCR primer/in situ hybridization probe | GTGCAGAACCGACCCGATG |
| Sequence-based reagent | Ctsk Rv primer | Bryant et al., 2017 | PCR primer/in situ hybridization probe | CAGCTGGACTCGGAGTGATGC |
| Sequence-based reagent | Kazald1 Fw primer | Bryant et al., 2017 | PCR primer/in situ hybridization probe | CTCGTGACATCCTGAGCCTGGAAG |
| Sequence-based reagent | Kazald1 Rv primer | Bryant et al., 2017 | PCR primer/in situ hybridization probe | GAAAATGGATAAGGTGGTGGGGAGGG |
| Sequence-based reagent | Krt17 Fw primer | Leigh et al., 2018 | PCR primer/in situ hybridization probe | CCTCTTGGACGTGAAGACC |
| Sequence-based reagent | Krt17 Rv primer | Leigh et al., 2018 | PCR primer/in situ hybridization probe | CCAGAGAAGATGAGCATACATCGG |
| Sequence-based reagent | Ctsk Fw primer | This paper | RT-qPCR primer | TGGCCCTTTTAACAACACCG |
| Sequence-based reagent | Ctsk Rv primer | This paper | RT-qPCR primer | ACTGAGTTGCAACAGCTTCC |
| Sequence-based reagent | Rpl4 Fw primer | This paper | RT-qPCR primer | TGAAGAACTTGAGGGTCATGG |
| Sequence-based reagent | Rpl4 Rv primer | This paper | RT-qPCR primer | CTTGGCGTCTGCAGATTTTTT |
| Sequence-based reagent | Trap Fw primer | This paper | RT-qPCR primer | TCATTGCCTGGTCAAGCATC |
| Sequence-based reagent | Trap Rv primer | This paper | RT-qPCR primer | TGGGCATAGTAGAACCGCAA |
| Sequence-based reagent | Dcstamp Fw primer | This paper | RT-qPCR primer | TGGAAACCAAAAGTGCAGCG |
| Sequence-based reagent | Dcstamp Rv primer | This paper | RT-qPCR primer | CCCCTCAGTGCCATCATTGT |
| Chemical compound, drug | Calcein | Sigma-Aldrich | Cat #C0875 | 0.1% solution |
| Chemical compound, drug | Alizarin red | Sigma-Aldrich | Cat #A5533 | 0.1% solution |
| Chemical compound, drug | Zoledronic acid | Sigma-Aldrich | Cat #SML0223 | Intraperitoneal (IP) injections, 200µg/kg |
| Commercial assay or kit | Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye | Invitrogen | Cat #C10337 | IP injections, 10µg/g |
| Commercial assay or kit | Masson’s trichrome staining | Sigma-Aldrich | Cat #HT15 | |
| Commercial assay or kit | pGEM-T Easy Vector Systems | Promega | Cat #A1360 | |
| Commercial assay or kit | RNAeasy Mini Kit | QIAGEN | Cat #74104 | |
| Software, algorithm | µManager | Edelstein et al., 2014 | https://micro-manager.org/ | |
| Software, algorithm | Fiji | Schindelin et al., 2012, Rueden, 2022 |
https://github.com/fiji/fiji RRID:SCR_002285 |
|
| Software, algorithm | Prism9 | GraphPad Software | https://www.graphpad.com | |
| Software, algorithm | Affinity Designer | Serif Europe | https://affinity.serif.com/ |