Figure 3.

Pink1 KO cortical neurons exhibit decreased spine density with relative increases in filopodia at the expense of mushroom spines, reversed by introduction of human PINK1. A, Representative images of primary and secondary dendrites in GFP-transfected neurons. Scale, 5 µm. B, Overall spine density. C–F, Density of filipodia, stubby spines, thin spines, and mushroom spines, respectively. G, Pie charts showing relative distribution of spine morphologies in the primary and secondary dendrites of WT versus KO cortical neurons with rescue in KO neurons transfected with hPINK1. Data in B–F are expressed as violin plots with the median and interquartile range indicated. Adjusted p values following post hoc Dunnett's T3 multiple comparisons test are shown (n = 26–39 neurons per condition compiled from three to four independent experiments; Table 2). ns - not significant.