Figure 6.

Pink1 KO mice show reduced spine densities in vivo. A, B, Representative coronal images of the somatosensory cortices of 6-month-old WT (A) and KO (B) littermates stained using the Golgi–Cox method. Scale bars: 200 μm. C, D, Neurons in the boxed areas of A and B are shown at higher magnification for (C) WT and (D) KO mice, respectively. Secondary apical dendrites are indicated with arrowheads. Scale bars: 50 μm. E, F, Representative images of secondary apical dendrites from a different pair of (E) WT and (F) KO mice. Scale bars: 10 μm. G, Quantification of spine density (n = 28–34 neurons compiled from two WT and three KO mice; p < 0.0001, t = 4.918 using Student's unpaired t test with Welch's correction).