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. 2022 Sep 26;610(7932):532–539. doi: 10.1038/s41586-022-05231-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — Pooled samples from 3 technical replicates. Ponceau S staining as a loading control. a, Sr35 NBD ATP-binding and coiled-coil protomer interface mutants. Myc-tagged protein. Left and right side merged from the same blot. b, Sr35 EDVID and arginine-cluster mutants. Myc-tagged protein. Last lane cropped from the same blot. c, Sr35 channel mutants. Myc-tagged protein. d, Sr35 LRR mutants. Myc-tagged protein. e, AvrSr35 mutants. YFP-tagged protein detected by GFP antibody.