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. 2022 Oct 12;610(7932):575–581. doi: 10.1038/s41586-022-05324-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Locus architecture and guide RNAs for IsrB (left) and Cas9 (right). b, Domain architecture of Streptococcus pyogenes SpCas9 (top) and D. thermocuniculi IsrB (DtIsrB) (bottom). c, Schematic of IsrB in complex with the ωRNA and the target DNA. The partial DNA duplex containing the TAM and target sequences used for the structural study are shown in sequence letters. d,e, Cryo-EM-density map (d) and structural model (e) of the IsrB–ωRNA-target DNA complex. Dashed lines represent poorly resolved regions of ωRNA. TE, transposon end; DR, direct repeat; NUC, nuclease; PI, PAM-interacting; PLL, phosphate-lock loop; TI, TAM-interacting; TS, target strand; NTS, non-target strand.