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. 2022 Oct 12;610(7932):575–581. doi: 10.1038/s41586-022-05324-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Cryo-EM data processing schematic for single particle analysis of the complex A. Unsharpened (Left) and sharpened (Right) maps in the final 3D refinement. Particle orientation distribution (Center). (b) Final refined map, colored by local resolution, calculated in RELION-4.0 with FSC threshold 0.5.(c) FSC curves calculated between the half maps of complex A from the final round of the refinement in RELION-4.0. (d) FSC curves calculated between the model and the final refined map, using phenix.validation_cryoem. (e) Q-scores for each residue of IsrB-ωRNA-target strand DNA-non-target strand DNA model in 3.1 Å map of the IsrB-ωRNA-DNA complex. The dashed black and grey lines in the plot represent the expected Q-scores based on the global map resolution (3.1 Å) and the local map resolution (4.5 Å), respectively. Q-scores for the RNA and DNA residues are consistent with the expected values based on the local map resolution.