FIG. 2.

Selected immunoblots from three adults (subjects E, F, and G) and a child (subject I), illustrating the patterns of anti-α-chain antibody-reactive bands obtained from samples of nasal secretions. Samples obtained by different methods from individual adults were collected during one day. The dilutions of individual samples in reducing sample buffer prior to analysis are indicated. Note that all samples display a prominent band corresponding to intact α chain (M_r, ∼58K). For interpretation of other bands, see the text. Lane 9 represents sample I_w,3 from the child, from which H. influenzae was cultured (Table 3). Numbers on the left indicate molecular weight standards in kilodaltons.