TABLE 2.
Immunoglobulin concentrations in 14 samples of nasal wash (obtained by method 3) from eight adults and 1 sample from a child as determined by isotype-specific ELISAa
| Subject and sampleb | Total IgA (μg ml−1) | S-IgA (%) | IgA1c (%) | IgG (μg ml−1) | IgM (μg ml−1) |
|---|---|---|---|---|---|
| A3,1 | 16.2 | 88 | 92 | 6.1 | 0.6 |
| B3,1 | 10.7 | 86 | 91 | 7.1 | 0.4 |
| B3,2 | 16.0 (458)d | NDe | 91 | 1.3 (37.2) | 0.4 (11.4) |
| C3,1 | 31.5 | 93 | 80 | 6.1 | 1.5 |
| D3,1 | 73.4 | 87 | 75 | 35.4 | 1.9 |
| E3,1 | 9.6 | 97 | 91 | 0.2 | 0.2 |
| E3,2 | 60.2 (1,336) | ND | 95 | 2.0 (44.4) | 1.1 (24.4) |
| E3,3 | 24.9 (553) | ND | 88 | 2.8 (62.2) | 0.4 (8.9) |
| F3,1 | 44.8 (896) | ND | 84 | 1.4 (28.0) | 0.3 (6.0) |
| F3,2 | 10.4 (231) | ND | 91 | 1.2 (26.6) | 0.1 (2.2) |
| G3,1 | 34.8 (870) | ND | 94 | 3.9 (97.5) | 0.4 (10.0) |
| G3,2 | 12.0 (343) | ND | 93 | 0.4 (11.4) | 0.1 (2.9) |
| G3,3 | 39.5 | ND | 91 | 1.0 | 0.3 |
| H3,1 | 116.3 (2,326) | ND | 93 | 5.5 (110) | 2.9 (58.4) |
| Iw,3f | 6.5 | ND | 89 | 0.2 | 0.05 |
| Median (range) | (711 [231–2,326]) | 88 [86–97] | 91 [75–95] | (40.8 [11.4–110]) | (9.5 [2.2–58.4]) |
IgA data include subclass distribution and, for five samples, estimates of S-IgA relative to total IgA. Calculated immunoglobulin levels in undiluted secretions are included for eight samples obtained with washing medium containing 2 mM lithium as an internal standard.
The first number indicates the method, and the second number indicates the sample.
Calculated as [IgA1] × ([IgA1] + [IgA2])−1 × 100%.
Numbers in parentheses indicate the concentration in the undiluted secretion (calculated for lithium-containing samples only).
ND, not determined.
Third sample of wash from the child (subject I).