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. 2022 Oct 19;13:6187. doi: 10.1038/s41467-022-33744-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Safranin-O staining and Osteoarthritis Research Society International (OARSI) scores of mouse knee joints of Runx2^fl/+ (R2-Hetero Cntl), Col2a1-Cre^ERT2; Runx2^fl/+ (R2-Hetero cKO), Runx2^fl/fl (R2-Homo Cntl), and Col2a1-Cre^ERT2; Runx2^fl/fl (R2-Homo cKO) mice 12 weeks after surgery to destabilize the medial meniscus (DMM) or 8 weeks after resection of the medial meniscus and medial collateral ligament (medial model). Tamoxifen induction was performed at 15 weeks in the DMM group and 7 weeks in the medial model group. Experimental groups consisted of n = 13, 14, 20, and 12 in the DMM model, respectively, and n = 16 per genotype in the medial model. Scale bars, 200 μm. Safranin-O staining and immunohistochemistry of Runx2, Sox9, Col2a1, and Mmp13 in sham (b) and surgical (c) joints of the medial model group. n = 6 biologically independent experiments. Scale bars, 100 μm. Data are expressed as dot plots and mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ^#P < 0.0001; ns, not significant; one-way ANOVA test with Turkey’s post hoc test.