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. 2022 Oct 19;13:6187. doi: 10.1038/s41467-022-33744-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a CisGenome browser screenshots showing Runx2 and reference engagement around Col2a1 (Chr15: 97,825,000 − 97,840,000) and Mmp13 (Chr9: 7,265,000 − 7,280,000). Lower panels show genomic regions cloned into the pGL4.10[luc] vector. b Luciferase activities of enhancer regions in mouse chondrocytes exposed to various concentrations of IL-1β. c, d Luciferase activities of enhancer regions in mouse chondrocytes transfected with various ratios of Runx2 and Sox9, or GFP control. e Luciferase activities of enhancer regions in chondrocytes from Runx2^fl/+ (R2-Hetero Cntl), Col2a1-Cre^ERT2; Runx2^fl/+ (R2-Hetero cKO), Runx2^fl/fl (R2-Homo Cntl), and Col2a1-Cre^ERT2; Runx2^fl/fl (R2-Homo cKO) mice with or without IL-1β exposure. n = 3 (b–e) biologically independent experiments. Data shown mean ± SD. ns not significant. ^#P < 0.05 vs control (white bars) (b), *P < 0.05 (c–e); one-way ANOVA test with Turkey’s post hoc test. RLA relative luciferase activity.