Fig. 9. Treatment with the 3D printed growth plate mimetic composite showed peripheral cartilage-like tissue in vivo.

Representative Alcian Blue Hematoxylin staining (i–iii) and Collagen type II staining (iv) of the proximal tibia growth plate of a an uninjured (Normal) or b untreated rabbit, as well as growth plates treated with c a fat graft, d gel only, or e Gel + 3D print. (i) overview ABH images (scale bar = 2000 μm) with the boxed areas shown as a higher magnification image (scale bar = 500 μm) in (ii) the central region of the growth plate and (iii) the periphery. C = cartilage, F = fibrous tissue and B = bone. (iv) Collagen type II immunohistochemistry at higher magnification (scale bar = 500 μm) near the periphery.