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. 2022 Oct 19;12:17440. doi: 10.1038/s41598-022-22156-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of claudin-3 augmented B16F10 melanoma lymphatic metastasis. (a) Schematic flowchart shows the establishment of B16F10 melanoma transplantation model in mice. (b) The tumor volume was measured and calculated after day 9 as described in the method part. (ns: no significance) (n = 5 mice per group) (c) mCherry fluorescence was detected by IVIS in ipsilateral popliteal lymph nodes (dpLN) of the footpad injection site from claudin-3^−/− and claudin-3^+/+ mice. (n = 5 mice per group) (d) Quantification of metastasized mCherry labeled B16F10 cells in dpLN was measured by IVIS. Statistical analysis of fluorescence was conducted. Values are shown as the mean ± SEM. (**p < 0.01) (n = 5 mice per group) (e) After sacrificing the mice, ipsilateral popliteal lymph node (dpLN), ipsilateral inguinal lymph node (diLN), liver, lung, and spleen were collected. Representative images show the fluorescence examined by IVIS.