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. 2022 Oct 19;12:17440. doi: 10.1038/s41598-022-22156-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of claudin-3 in SVEC4-10 cells inhibits cell migration. (a) SVEC4-10 cells were transfected with claudin-3 plasmid to overexpress the protein accompanied with the transfection of control plasmid. RT-PCR analysis was used to examine the mRNA expression in the overexpression and control cells. (***p < 0.001) (n = 3 per group) (b) Western blot analysis indicated the overexpression of claudin-3 protein in SVEC4-10 cell. (c) Representative images of tube formation of SVEC4-10 cells transfected with control and claudin-3 overexpression plasmids. Scale bar, 100 μm. (d) Number of junctions and total length were analyzed as indicators of tube formation ability via the ImageJ. (*p < 0.05, **p < 0.01) (n = 3 per group) (e) Scratching assay was conducted and the photos were taken under a microscope. Scale bar, 100 μm. Wound width was quantified and analyzed by t-test. (**p < 0.01) (n = 5 per group) (f) Transwell assay was conducted and the representative images were taken. Scale bar, 100 μm. Cells on the bottom side were counted and analyzed by t-test. (**p < 0.01) (n = 5 per group).