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. 2022 Oct 19;12:17440. doi: 10.1038/s41598-022-22156-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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VEGF-C is sufficient to downregulate claudin-3 expression on SVEC4-10 cells. (a) SVEC4-10 cells were co-cultured with conditional medium (CM) from B16F10 cells for the indicated time. RT-PCR analysis was used to examine the mRNA expression of claudin-3. (***p < 0.001) (n = 3 per group) (b) Western blot analysis indicated the protein expression level of claudin-3 protein in SVEC4-10 cell. (c) Quantitative analysis for western blot results. (**p < 0.01, ***p < 0.001) (n = 3 per group) (d) SVEC4-10 cells were treated with 0 ng/mL, 50 ng/mL or 100 ng/mL VEGF-C for 12 h or 24 h. RT-PCR analysis was used to examine the mRNA expression of claudin-3. (*p < 0.05, **p < 0.01) (n = 3 per group) (e) Western blot analysis indicated the protein expression level of claudin-3 protein in SVEC4-10 cell. (f) Quantitative analysis for western blot results. (**p < 0.01, ***p < 0.001) (n = 3 per group) (g) Wound healing assay of SVEC4-10 cultured with CM from the B16F10 cells and normal control. The ratio of CM to fresh complete medium was 1:3. Photos were taken at the beginning and 16 h later. Scale bar, 100 µm. (h) Wound width was quantified and analyzed by t-test. (*p < 0.05, ***p < 0.001) (n = 5 per group).