Fig. 3. The metabolic landscape is differently modified in young and aged animals fed HGD.

a Heat maps of glucose and lipid metabolism genes. b Quantification and representative immunoblot of GFP levels in day 10 far-3p::gfp animals fed ND, HGD-1 and HGD-5 (n = 4). c Major glucose metabolic pathways in C. elegans, f6p fructose-6-phosphate, g1p glucose-1-phosphate, g6p glucose-6-phosphate, HP hexosamine pathway, PPP pentose phosphate pathway, UDP-G uridine diphosphate glucose. Adapted from Seo et al.⁸⁸ and created with BioRender.com. d Trehalose levels of worms treated as in WT, HGD-1 and HGD-5 at day 10 (n = 3). e Representative images of Oil Red O (ORO) staining of ND, HGD-1 and HGD-5 animals harvested on day 10. Lipid droplets were quantified from the green channel within regions of interest (yellow-dashed circles) as previously reported⁵⁵. Scale bar represents 100 μm. f Quantification of ORO-stained lipid droplets at the pharynx of animal as in e (ND, n = 127; HGD-1, n = 110; HGD-5, n = 112 including biological replicates). Data shown are the mean ± SEM. Statistical analysis was subjected to one-way ANOVA with Tukey’s test.