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. 2022 Oct 6;13:1009198. doi: 10.3389/fimmu.2022.1009198

Figure 4.

Figure 4

Generation of bone marrow chimeras of the clones in thymoproteasome deficient mice. WT or Psmb11-/- mice were sub-lethally irradiated and transplanted with hematopoietic stem cells transgenic for the indicated clones. Eight weeks later, thymi and LNs were analyzed by flow cytometry. n = 4-8 mice per group from three independent experiments. (A) Representative FACS plots showing thymic mature SP8 for all of the clones in WT or Psmb11-/- mice. (B) Numbers of donor mature SP8 in thymi of WT or Psmb11-/- mice. Mean. n = 4-5 mice per group from two independent experiments. (C) Numbers of donor CD8+ T cells isolated from thymi of WT or Psmb11-/- mice depicted as a ratio between WT and Psmb11-/- (KO). Median. The statistical analysis was performed using the two-tailed Mann Whitney U test. (D) A linear fit between the WT : KO ratio of mature SP8 cells and CD5 gMFI levels. (E) Numbers of donor CD8+ T cells isolated from LNs of WT or Psmb11-/- mice. n=4-8 mice per group from three independent experiments. The statistical analysis was performed using Kruskal–Wallis test with Dunn’s post-tests. (F) Numbers of donor CD8+ T cells isolated from LNs of WT or Psmb11-/- mice depicted as a ratio between WT and Psmb11-/- (KO). Median. The statistical analysis was performed using the two-tailed Mann Whitney U test. ** p<0.01, ns (not significant) p>0.05.