| Type | Mechanism | Characteristic |
|---|---|---|
| Competitive-type | Only bound free enzyme and not the enzyme–substrate complex |
1. Ki ≈ Kis; 2. The lines of Lineweaver–Burk are crossed on a 1/V axis |
| Noncompetitive-type | Only bound the enzyme–substrate complex |
1. Ki ≈ Kis; 2. The lines of Lineweaver–Burk crossed on a 1/[S] axis |
| Mixed-type | Bound free enzyme and the enzyme–substrate complex |
1. The lines of Lineweaver–Burk crossed on crossed on the second or third quadrant; 2. Ki < Kis, competitive-type; 3. Ki > Kis, noncompetitive-type |
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