Figure 4.

UCP2 knockdown decreases cell proliferation and has a selective impact on respiration in T-ALL cell lines. Immunoblot of UCP2 in whole cell extracts from either parental (n = 3) or UCP2^CRISPR (n = 3) Jurkat (A) and HPB-ALL (B) after 72 hours of incubation in complete medium (+) and glutamine-deprived medium (–); quantification of UCP2 expression normalized to β-actin and expressed as a mean ± SEM; significance: *** = p<0.001 * = vs. complete medium (+) parental cells. (C) Doubling time in hours as determined during 72 hours of culture in complete or glutamine-deprived medium (-GLN) for either parental or UCP2^CRISPR Jurkat and HPB-ALL; black bars = parental Jurkat cells; white bars = UCP2^CRISPR Jurkat cells; dark gray bars = parental HPB-ALL cells; light gray bars = UCP2^CRISPR HPB-ALL cells; data are expressed as mean ± SEM (n = 4). (D) The basal energy metabolism was assessed by analyzing the OCR/ECAR ratio measurement with SeaHorse XF96 equipment respectively in complete medium (complete) and in glutamine-deprived medium (-GLN), for either parental or UCP2^CRISPR Jurkat and HPB-ALL; black bars = parental Jurkat cells; white bars = UCP2^CRISPR Jurkat cells; dark gray bars = parental HPB-ALL cells; light gray bars = UCP2^CRISPR HPB-ALL cells; data are expressed as mean ± SEM (n = 4). Statistical significance is represented as follows: ***p<0.001; **p<0.01; *p<0.05. ns, not significant.