Figure 4.

Anti-apoptotic effect of FGF1 stimulation in U2OSR1 cells treated with drugs targeting tubulin polymerization. (A) Relative caspase 3/7 activity induced by 5 nM TLT in U2OSR1 cells was measured using ApoLive-Glo Multiplex Assay 24 h after drug administration in the presence or absence of 10 ng/mL FGF1 and 100 nM PD173074. Results represent the mean ± SD of at least three independent experiments and are normalized to untreated cells; statistical significance: **p<0.01, no significant difference is marked as ‘ns’. (B) Protective effect of FGF1 against drug-induced apoptosis in U2OSR1 cells assessed by PARP cleavage. Western blotting was performed with anti-PARP antibodies 24 h after administration of 5 nM TLT, 20 nM PTX or 10 nM VCR in the presence or absence of 10 ng/mL FGF1.