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. 2022 Oct 6;12:1011762. doi: 10.3389/fonc.2022.1011762

Figure 5.

Figure 5

Effect of AKT inhibition on protective effect of FGF1 against TLT. Viability of U2OSR1 cells treated with 5 nM TLT and (A) different chemical inhibitors of major FGF-induced signaling pathways (20 µM LY294002 (PI3K), 20 µM UO126 (MEK1/2), 5 µM SB203580 (p38), 100 nM Torin-2 (mTOR)) or (B) FGFR inhibitor (100 nM PD173074), a direct AKT inhibitor (1 µM API-2), a dual mixture of PI3K and mTOR inhibitors (20 µM LY294002 + 100 nM Torin-2) or an inhibitor of both kinases, PI3K and mTOR (100 nM BEZ235) for 48 h in the presence or absence of 10 ng/mL FGF1, monitored by the alamarBlue assay. Results represent the mean ± SD of at least three independent experiments and are normalized to cells untreated with TLT; statistical significance: *p<0.05, **p<0.01, ***p<0.001, no significant differences are marked as ‘ns’.