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. 2022 Oct 6;10:1001606. doi: 10.3389/fcell.2022.1001606

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Copyright © 2022 Ge, Shentu, Wang, Wang, Xue, Yue, Mao, Zhang, Xu, Li, Ma, Yao, Cui and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of angiogenesis-associated risk genes in PC: (A–D) Survival analyses of four signature genes; (F–H) The histological expression of four signature genes from HPA database. The top of the figure indicates the category of tissue specimen. The name of angiogenesis gene, the antibody type used in immunohistochemistry, and the patient ID of tissue specimens are shown at the bottom of each image; (I–L) qRT-PCR analyses of the mRNA expression levels of angiogenesis-associated risk genes in PC cell lines (BXPC-3, CFPAC-1, and PANC-1) and normal pancreas cells (HPNE). (M–P) Correlation of angiogenesis-associated risk genes with tumor metastasis in PC. ****p < 0.0001 by repeated measures with two-tailed unpaired Student’s t-test.