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. 2022 Oct 20;60(4):2102347. doi: 10.1183/13993003.02347-2021

FIGURE 4.

FIGURE 4

Pirfenidone leads to decreased vascular endothelial (VE)-cadherin expression in the lungs of Fra-2-overexpressing transgenic (Fra-2 TG) mice. a) Schematic of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway “Leukocyte transendothelial migration”. The log fold change of gene expression is indicated by colour, significance of regulation is indicated by box borders. b) Quantitative real-time PCR analysis of VE-cadherin (Cdh5). Data are presented as boxplots with dot-plot overlays. Statistical analysis was performed using nonparametric Kruskal–Wallis testing with post-analysis to compare specific groups. *: p<0.05, **: p<0.01, ***: p<0.001. c) Low- and high-magnification immunofluorescence images of VE-cadherin (Cdh5) and von Willebrand factor (Vwf) staining in lung tissue from wild-type (WT) and Fra-2 TG mice with (+P) and without pirfenidone treatment. Nuclear counterstain was performed using 4′,6-diamidino-2-phenylindole (DAPI). Scale bars=10 µm. d) Spearman's rank correlation analysis of inflammatory cells in the bronchoalveolar lavage fluid (BAL cell count) and Cdh5 expression.