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. 2022 Oct 6;13:978584. doi: 10.3389/fneur.2022.978584

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Copyright © 2022 Kim and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro measurement of vacuum pressure. (A) The pump was connected to a pressure gauge using a three-way pressure gauge inline adapter (luer lock, ISO 594-2); additionally, the system was completely closed by stopcock, which is attached at the end of the three-way adapter. (B) A 60cc syringe was connected to a pressure gauge, and a plunge was locked into place.