Figure 4.

In vivo prevascularized macroporous β-TCP scaffold enhanced bone regeneration within the tibial defect site (A, B) Regenerated bone tissue in histological sections through the segmental tibia defect of rats implanted with either a blank or prevascularized scaffold. The sections were stained with hematoxylin and eosin (A) or Masson's trichrome stain (B) 2, 4, 6, and 8 weeks after implantation. New generated bone is marked with arrows in black and host bone tissue with white arrows in HE staining. n ​= ​3 in each group. Higher magnification photographs of boxed areas are shown in the subjacent panels. Scale bars, 400 ​μm.