Table 3.
Flow cytometry definition of BM cell populations. Distribution of the main BM cell populations, according to their immunophenotype.
| Type of Cells | Immunophenotype1 | Median (range) |
|---|---|---|
| Immature granulocytes | SSC++/FSC++; CD45dym/+/CD16low/CD10neg | 22.85% (0.4-57.2) |
| Mature granulocytes | SSC++/FSC++; CD45dym/CD16++/CD10++ | 12.6% (0.2-57.8) |
| IG/N ratio | na2 | 1.26 (0.02-5.2) |
| Mast cells | SSC++/FSC++; CD117hi/CD45dym | 0.006% (0-0.11) |
| Plasma cells | SSClow/FSC+; CD138hi | 0.2% (0-3) |
| Hematogones | SSClow; CD81hi/CD10+/CD45dym | 0.9% (0-9) |
| B lymphocytes | SSClow; CD45hi/CD19+/CD56neg | 1.6% (0-10) |
| NK-like T cells/NK | SSClow; CD45hi/CD19neg/CD56hi | 0.4% (0.3-16) |
| Erythroblasts | SSClow/FSClow; CD45neg/CD71+ | 5.3% (0.4-12.4) |
1According to the EuroFlow consortium; 2Not applicable (na).