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. 2022 Aug 18;33(10):ar89. doi: 10.1091/mbc.E21-12-0603

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© 2022 Kanagy et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — ITAM phosphorylation by an SFK is required before Syk recruitment. (A) Syk(SH2)^SYFP2, which retains both SH2 domains but lacks the kinase domain, is recruited to FcεRI aggregates in RBL-Lyn/Syk^dKO. Representative image from 50 cells imaged. (B) Pretreatment for 30 min with 0.1 mM dasatinib (Das.) prevents Syk(SH2)^SYFP2 recruitment. Representative image from 73 cells imaged. Quantification of Syk(SH2)^SYFP2 recruitment frequency is found in Supplemental Figure S1. Scale bars, 5 µm.