TABLE 1:
Thermodynamic parameters of interaction between hSurvivin and histone H3 peptides at pH 7.2 at 25°C.
| Peptide | N | Kd [µM] | ΔH (cal/mol) | –TΔS (cal/mol) |
|---|---|---|---|---|
| H3T3phK4me1(1-12) | 1.0301 ± 0.005 | 3.10 ± 0.14 | –4692 ± 26 | –2820 |
| H3T3phK4me2(1-12) | 0.873 ± 0.004 | 2.82 ± 0.16 | –6250 ± 60 | –1310 |
| H3T3phK4me3(1-12) | 1.21 ± 0.02 | 11.5 ± 1.0 | –4630 ± 90 | –2820 |
| H3K4me1(1-12) | 1.080 ± 0.012 | 9.1 ± 1.0 | –2680 ± 60 | –4200 |
| H3K4me2(1-12) | 0.899 ± 0.010 | 12.0 ± 0.8 | –2610 ± 40 | –4110 |
| H3K4me3(1-12) | 0.83 ± 0.07 | 87 ± 10 | –6000 ± 700 | –500 |
| H3T3phK4ac(1-12) | 0.965 ± 0.009 | 15.4 ± 0.8 | –7300 ± 100 | –700 |
| H3T3ph(1-12) | 1.03 ± 0.002 | 2.31 ± 0.08 | –4880 ± 30 | –2800 |
| H3(1-12) | 1.21 ± 0.01 | 9.37 ± 0.31 | –2790 ± 20 | –4040 |
Measurements were performed in 25°C in a triple component buffer containing citric acid, HEPES, CHES, and 10 mM β-mercaptoethanol. N, stoichiometry; Kd, dissociation constant; ΔH, entalphy change; ΔS, entropy change; T, temperature. Values for H3T3ph(1-12) and H3(1-12) were taken from Niedzialkowska et al. (2012).