REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
α-Tubulin-AF555 (mouse)	EMD Millipore	Cat# 05-829-AF555
CDK5RAP2/Cep215 (rabbit)	Bethyl laboratories	Cat# IHC-00063, RRID: AB_2076863
Cep192 (rabbit)	Bethyl laboratories	Cat# A302-324A,
Centrin (mouse)	EMD Millipore	Cat# 04-1624, RRID: AB_10563501
DAPI	SigmaAldrich	Cat# D9542-10MG
Donkey anti-mouse AF647	Fisher Scientific	Cat# A31571, AB_162542
Donkey anti-rabbit AF 488	Life Technologies	Cat# A21206, RRID: AB_2535792
Donkey anti-rabbit AF 568	Life Technologies	Cat# A10042, AB_2534017
Donkey anti-rabbit AF 647	Life Technologies	Cat# A31573, RRID: AB_2536183
Gamma-tubulin (mouse)	Abcam Biochemicals	Cat# 11316, RRID: AB_297920
Gamma-tubulin (rabbit)	Sigma Aldrich	Cat# T5192, RRID: AB_261690
Pericentrin (rabbit)	Abcam Biochemicals	Cat# ab4448 AB_304461
PLK1 (rabbit)	Cell Signaling Technology	Cat# 4513S, RRID: AB_2167409
Phospho-PLK1 (Thr210) (rabbit)	Cell Signaling Technology	Cat# 5472, RRID: AB_10698594
Phospho-(Ser/Thr) (rabbit)	Cell Signaling Technology	Cat# 9631, RRID: AB_330308
ODF2/cenexin (rabbit)	Thomas Scientific	Cat# 12058-1-AP
Chemicals, Peptides, and Recombinant Proteins
Acrylamide solution, 40%	Sigma Aldrich	Cat# A4058-100ML
Acrylamide-bisacrylamide solution	EMD Millipore	Cat# 1300-500ML
Agarose	Thermo Fisher	Cat# 16520100
APS (ammonium presulfate)	Fisher Bioreagents	Cat# BP179-100
BI2536	Selleck Chemicals	Cat# S1109
Bovine serum albumin	Fisher Scientific	Cat# BP1600-100
Dimethylsulfoxide	Fisher Scientific	Cat# BP231100
DABCO (1,4-diazabicyclo [2.2.2] octane, 97%)	Fisher Scientific	Cat# AC112470250
EDTA	Fisher Scientific	Cat# BP120-500
NaCl	Fisher Bioreagents	Cat# BP358-212
Nocodazole	Fisher Scientific	Cat# AC358240500
Paraformaldehyde	Fisher Scientific	Cat# AA433689M
Phosphate-buffered saline	Fisher Scientific	Cat# 10010023
Prolong gold	Invitrogen	Cat# P36934
Sodium acrylate, 97%	Sigma Aldrich	Cat# 408220-25G
ProTAME	R&D Systems	Cat# I-440-01M
TEMED	Fisher Scientific	Cat# BP150-100
Tris base	Fisher Bioreagents	Cat# BP152-5
Triton X-100	Fisher Scientific	Cat# BP151500
Tween 20	Thermo Fisher	Cat# BP337500
Experimental Models: Organisms/Strains
Human HeLa cells control shRNA	Hung et al., 2016	N/A
Human HeLa cells cenexin shRNA	Hung et al., 2016	N/A
Zebrafish	Zebrafish International Resource Center (ZIRC)	TAB (wild type)
Zebrafish	Gift from Solnica-Krezel Lab, generated by Harris Lab	Tg(-5actb2:cetn4-GFP)
Software and Algorithms
Adobe Illustrator
ImageJ/FIJI	Schindelin et al., 2012	https://imagej.net/Fiji
Prism8	GraphPad	https://www.graphpad.com/scientific-software/prism/
LAS-X software	Leica microsystems	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
R version 4.1.2; package ggtree	The R project for statistical computing	https://www.r-project.org