Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 7;33(11):ar99. doi: 10.1091/mbc.E21-11-0585

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Hansen et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License.

PMC Copyright notice

FIGURE 5: — Fully automated analysis of cellular morphology in tissue sections. (a) Maximum-intensity projection of a confocal z stack of Iba1-labeled murine cortical microglia. Scale bar, 50 µm. (b) Maximum-intensity projection of automatically detected cells after analyzing (a) with MotiQ 3D analyzer (using a high size threshold [of 10,000 voxels] to reconstruct cells that are moreover complete in the stack). (c) MotiQ visualizations of each cell: reconstructed cell (left), spanned volume (middle), and cell skeleton (right). Scale bars, 20 µm. (d, e) Direct comparison of the results obtained with MotiQ (c) to results obtained using (d) an alternative automated approach based on the commercial software Imaris or (e) a manual approach using the Fiji plug-in Simple Neurite Tracer (Arshadi et al., 2021). Each data point represents one cell. MotiQ results are indicated on the x-axis. Imaris (d) or manual tracing (e) results are indicated on the y-axis. Lines show mean and 95% confidence interval of a linear regression. r and p values for a Pearson correlation are indicated. Note: Imaris and the manual approach did not allow detection of the top right cell in the image (a), as the cell body is not completely within the stack. Thus, this cell is missing in the correlations.