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. 2022 Sep 7;33(11):br18. doi: 10.1091/mbc.E22-05-0150

FIGURE 4:

FIGURE 4:

Loss of BB connections does not inhibit ciliary beat frequency. (A) Schematic image depicting ciliary beat frequency quantification using kymograph analysis. The X-axis represents time (total duration: 105 ms). The Y-axis represents distance. Scale bar, 2 µm. (B) Kymographs of WT and disA-1 cilia. disA-1 cilia display variable ciliary trajectories between ciliary beat cycles (red arrowheads). Scale bar, 2 µm. (C) Quantification of WT and disA-1 ciliary beat frequency of individual cilia. Three cilia per cell were sampled. disA-1 cilia exhibit ciliary beat frequencies comparable to those of WT cilia. WT: n = 28 cells (84 cilia). disA-1: n = 38 cells (72 oriented cilia; 41 disoriented cilia). Mann–Whitney test. Mean ± SD. (D) Duration of power and recovery strokes of WT and disA-1 cilia. WT: n = 11 cells (25 cilia). disA-1: n = 13 cells (32 cilia). Mann–Whitney test. F test for variance comparison. Mean ± SD. (E) Schematic illustrating comparable average durations of ciliary beat cycle for WT and disA-1 cilia. disA-1 cilia beat at a ciliary beat frequency comparable to that of WT cilia with a longer power stroke duration and a shorter recovery stroke duration.