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. 2022 Sep 7;33(11):ar94. doi: 10.1091/mbc.E21-11-0537

FIGURE 4:

FIGURE 4:

GI-SIM shows single-filament severing. (A) Five panels from Supplemental Movie 1 show filament severing. Hollow yellow and magenta arrowheads in i show a top and bottom filament that become stretched in ii. Both filaments are severed between ii and iii, and the bottom filament leaves the image plane. Hollow yellow arrowheads in iii–v point to the filament ends that spring apart under tension. (B, C) Two additional severing events; magenta arrowheads show the filaments before (panels i) and after (panels ii–v) severing. Filament breakage is also shown in Supplemental Movie 8.