Movie S7.
Filament dynamics in an anisotropically contracting and expanding amnioserosa cell. 4 movie panels relate pulsations in cell area (area approximated by the location of actin-rich protrusions emanating from cell junctions), filament orientation, the overall orientation of the cell and polar histograms of the orientation of the filaments. (Left) GI-SIM images of F-actin in an amnioserosa cell from a Lifeact-mEGFP labeled embryo (approximate boundary shown with a dashed yellow outline). (Middle) Traces of the actin filaments from the left panel. (Right upper) Cell area (black), major (red) and minor (blue) axes of ellipses fit to the cell area shown as a function of time in the cell outlines from left panel. (Right lower) Polar histograms of filament orientation as a function of time. The movie in Left panel is from a time-lapsed sequence sped up 30 times. Animations of filament traces, graphs of area, major and minor ellipse axes and polar histograms correspond in time to the movie. Stills are shown in Figure 3.