TABLE 2.
List of functionally annotated proteins from ORFs in the genome of phage JJ01.
| ORF | Predicted function | Direction | Start | Stop | Size (n) | Sequence similarity | Accession no. | Database |
| ORF7 | Head morphogenesis protein | – | 2533 | 3423 | 891 | PHAGE_Pseudo_vB_PaeM_E217 _NC_042079: head morphogenesis protein |
PP_00007 | PHASTER |
| ORF8 | Terminase large subunit | – | 3584 | 4771 | 1188 | PHAGE_Pseudo_vB_PaeM_SCUT _S1_NC_048745: terminase large subunit |
PP_00008 | PHASTER |
| ORF10 | Terminase small subunit | + | 5349 | 6134 | 786 | PHAGE_Pseudo_vB_PaeM_E217 _NC_042079: terminase small subunit |
PP_00009 | PHASTER |
| ORF13 | DNA primase | + | 7853 | 8929 | 1077 | RepB family DNA primase [Staphylococcus aureus] | WP_015994935.1 | NCBI |
| ORF15 | DNA helicase | + | 9268 | 11007 | 1740 | DNA helicase [Pseudomonas phage Epa20] | QIQ65309.1 | NCBI |
| ORF17 | Minor tail protein | – | 12714 | 13325 | 612 | PHAGE_Pseudo_vB_PaeM_LS1 _NC_048699: minor tail protein |
PP_00016 | PHASTER |
| ORF18 | IgA FC receptor precursor | – | 13514 | 14200 | 687 | IgA FC receptor precursor [Pseudomonas phage debbie] | QIQ66578.1 | NCBI |
| ORF23 | Exonuclease | - | 15478 | 16122 | 645 | PHAGE_Pseudo_vB_PaeM_LS1 _NC_048699: exonuclease |
PP_00022 | PHASTER |
| ORF30 | DNA adenine methyltransferase | – | 17616 | 17804 | 189 | PHAGE_Pseudo_vB_PaeM_LS1 _NC_048699: DNA adenine methyltransferase |
PP_00030 | PHASTER |
| ORF31 | Tail length tape-measure protein | – | 17807 | 18493 | 687 | Tail length tape-measure protein [Pseudomonas phage debbie] | QIQ66591.1 | NCBI |
| ORF32 | Methyltransferase type 11 | – | 18546 | 18848 | 303 | PHAGE_Pseudo_vB_PaeM_SCUT _S1_NC_048745: methyltransferase type 11 |
PP_00032 | PHASTER |
| ORF34 | Putative phosphoesterase | – | 19203 | 19367 | 165 | PHAGE_Pseudo_vB_PaeM_LS1 _NC_048699: putative phosphoesterase |
PP_00035 | PHASTER |
| ORF36 | Putative terminase | + | 19772 | 21154 | 1383 | Putative terminase [Pseudomonas phage KPP12] | YP_007238156.1 | NCBI |
| ORF41 | DUF2786 protein | – | 22569 | 23348 | 780 | [pfam10979] cl12553 (PSSM Id: 402523) Protein of unknown function | cl12553 | NCBI Conserved Domain Search |
| ORF42 | Holin | – | 23435 | 23872 | 438 | PHAGE_Pseudo_Epa13 _NC_050147: holin |
PP_00043 | PHASTER |
| ORF50 | Minor capsid protein | + | 27664 | 29961 | 2298 | Minor capsid protein [Pseudomonas phage vB_PaeM_USP_1] | YP_009914222.1 | NCBI |
| ORF51 | Phage Mu protein F like protein | + | 29961 | 30797 | 837 | [Phage_Mu_F super family] cl10072 (PSSM Id: 415838) Phage Mu protein F like protein | cl10072 | NCBI Conserved Domain Search |
| ORF54 | DUF2213 protein | + | 31672 | 33105 | 1434 | [DUF2213 super family] cl19842 (PSSM Id: 418671) Uncharacterized protein conserved in bacteria | cl19842 | NCBI Conserved Domain Search |
| ORF56 | DUF2184 protein (major capsid) | + | 33754 | 34902 | 1149 | [DUF2184 super family] cl21556 (PSSM Id: 419730) Uncharacterized protein conserved in bacteria | cl21556 | NCBI Conserved Domain Search |
| ORF58 | DUF4054 domain-containing protein | + | 35456 | 35923 | 468 | DUF4054 domain-containing protein [Pseudomonas aeruginosa] | WP_016066139.1 | NCBI |
| ORF59 | Putative structural protein | + | 35920 | 36318 | 399 | Putative structural protein [Staphylococcus aureus] | WP_016066140.1 | NCBI |
| ORF60 | Structural protein | + | 36326 | 36877 | 552 | Structural protein [Pseudomonas phage phiKTN6] | YP_009593265.1 | NCBI |
| ORF62 | DUF3383 domain-containing protein | + | 37471 | 38985 | 1515 | DUF3383 domain-containing protein [Staphylococcus aureus] | WP_174840936.1 | NCBI |
| ORF67 | Putative structural protein | + | 40609 | 41112 | 504 | Putative structural protein [Pseudomonas phage KPP12] | YP_007238188.1 | NCBI |
| ORF68 | Structural protein | + | 41247 | 41651 | 405 | Structural protein [Pseudomonas phage goonie] | QJB23037.1 | NCBI |
| ORF71 | Transglycosylase SLT domain-containing protein | + | 42695 | 45271 | 2577 | Transglycosylase SLT domain cl00222 (PSSM Id: 396169) | cl00222 | NCBI Conserved Domain Search |
| ORF74 | Gp138 N-terminal domain-containing protein | + | 46723 | 47388 | 666 | [Gp138_N super family] cl39697 (PSSM Id: 423479) Phage protein Gp138 N-terminal domain | cl39697 | NCBI Conserved Domain Search |
| ORF75 | Baseplate J/gp47 family protein | + | 47446 | 48699 | 1254 | Baseplate J/gp47 family protein [Pseudomonas aeruginosa] | MBI7739469.1 | NCBI |
| ORF76 | DUF2612 protein | + | 48696 | 50210 | 1515 | [DUF2612 super family] cl12607 (PSSM Id: 416601) Protein of unknown function | cl12607 | NCBI Conserved Domain Search |
| ORF79 | Putative endolysin | + | 53539 | 54201 | 663 | Putative endolysin [Pseudomonas phage KPP12] | YP_007238200.1 | NCBI |
| ORF81 | DNA ligase | – | 54757 | 55668 | 912 | DNA ligase [Pseudomonas phage PHW2] | QKW95294.1 | NCBI |
| ORF82 | DNA-binding protein | – | 55723 | 56277 | 555 | DNA-binding protein [Pseudomonas phage debbie] | QIQ66549.1 | NCBI |
| ORF85 | U-spanin | – | 57924 | 58544 | 621 | PHAGE_Pseudo_vB_PaeM_E215 _NC_042080: u-spanin |
PP_00086 | PHASTER |
| ORF86 | SNF2 family N-terminal domain-containing protein | – | 58639 | 60198 | 1560 | [SNF2_N Superfamily] cl37620 (PSSM Id: 422040) SNF2 family N-terminal domain |
cl37620 | NCBI Conserved Domain Search |
| ORF87 | Putative DNA helicase | – | 60195 | 60605 | 411 | Putative DNA helicase [Pseudomonas phage NH-4] | YP_007002602.1 | NCBI |
| ORF88 | DNA polymerase III subunit alpha | – | 60598 | 63708 | 3111 | DNA polymerase III subunit alpha [Staphylococcus aureus] | WP_174840946.1 | NCBI |
| ORF89 | Putative DNA polymerase III epsilon subunit | – | 63705 | 64259 | 555 | Putative DNA polymerase III epsilon subunit [Pseudomonas phage NH-4] | YP_007002604.1 | NCBI |
| ORF92 | Putative thymidylate synthase | – | 65550 | 66338 | 789 | Putative thymidylate synthase [Pseudomonas phage PaGU11] | YP_009913899.1 | NCBI |
The predicted functions of ORFs were determined by their significant hit (E-value < 10–5) against the genome in NCBI and PHASTER.