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Analysis of IGFBP3 expression in H1299 cells.
pIRES2-EGFP-IGFBP3 was transfected into H1299 cells. At 24 h post-transfection, IGFBP3 was analyzed (a) by Western Blot in H1299 lysates and supernatants, and (b) by ELISA in supernatants. (c) The transfection rate was determined by flow cytometry, and (d) the function of secreted IGFBP3 was validated by its ability to bind free IGF-1. Two-sided P-value < 0.05 was considered statistically significant (n = 7; *P < 0.05).
