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Influence of IGFBP3 on MMP expression and activity.
IGFBP3 transfected H1299 cells were seeded in 6-well culture plates (75 × 103/well). After 72 h, concentration of (a) MMP-1; (b) MMP-9, and (c) TIMP-1 in supernatants was measured by ELISA. (d) The total MMP activity in supernatants was determined by MMP activity assay. Two-sided P-value < 0.05 was considered statistically significant (n = 7; *P < 0.05).
