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. 2022 Aug 5;37(5):676–684. doi: 10.1016/j.virs.2022.07.013

Fig. 2.

Fig. 2

EV71 infection induces OAS3 but not RNase L expression by IFN pathway. EV71 infection upregulates the expression of OAS3 but not RNase L. HEK293T cells were infected with EV71 at an MOI of 4, and the expression levels of OAS3 and RNase L were examined by Western blotting (A). The mRNA levels of OAS3 and RNase L were examined by RT-qPCR (B). The RNA level of “0 ​h” was set as “1”. C Construction of IFNAR1 CRISPR/Cas9 knockdown cell line. The efficiency of knockdown was examined by Western blotting. D The negative control pLKO.1 and IFNAR1 knockdown (IFNAR1 KD) HEK293T cells were treated with 250 U/mL IFN-β1b for 48 ​h, and the expression levels of MxA and ISG15 were examined by RT-qPCR at different time points. The RNA level of “0 ​h” was set as “1”. E IFNAR1 KD HEK293T cells were infected with EV71 at an MOI of 4, and the expression levels of OAS3 and RNase L were examined by Western blotting. F The mRNA levels of OAS3 and RNase L were examined by RT-qPCR. The RNA level of “0 ​h” was set as “1”. The results represent the means ​± ​standard deviation from three independent experiments. Statistical significance was analyzed using Student's t-test (# no significance, ∗∗P ​< ​0.01).