Fig. 3.

UCP2 overexpression decreases the number of IHC synapses and IHCs. A, C and E Representative images of IHC synapses (CtBP2, red) in the apical, middle and basal turns of the cochlea, respectively. IHCs and AAV-ie vector were labeled with anti-Myosin VIIa (grey) and anti-GFP (green), respectively. Scale bar, 20 μm. B, D and E Quantification the number of IHC synapses in the apical, middle and basal regions of the cochlea, respectively (n = 5 ears/group). G, H Percentage of survival OHCs and IHCs in the apical, middle and basal turns of the cochlea (n = 5 ears/group). UCP2 uncoupling protein 2, IHC inner hair cell, n.s. no significance, *p < 0.05, **p < 0.01