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. 2022 Oct 19;23:437. doi: 10.1186/s12859-022-04984-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — PC analysis, overall survival rate and analysis of TME of LC in 2 robust clusters. A The optimal cluster number was two using the ConsensusClusterPlus package. B Principal component analysis (PCA) was also an intuitive way to assess the optimal k. C Overall survival analysis between cluster 1 and cluster 2 of LC. D–F Comparison of stromal score, immune score and estimate score between two clusters in the TME of LC samples. G Violin diagram of the proportion of 22 types of immune cells involved in two clusters in LC samples. H The infiltration of immune cells involved in cluster1 and cluster2 in LC samples with 7 algorithm. *p < 0.05; **p < 0.01; ***p < 0.001