Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 20;41:307. doi: 10.1186/s13046-022-02518-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — The identification and characteristics of circLRFN5 in GBM. a, b Heatmaps (a) and volcano plots (b) of circRNAs that were differentially expressed between GBM and normal brain tissues (NBT) in GSE109569. c The expression of circLRFN5 in GBM tissues and NBT as measured by qPCR. d Schematic illustration of the genomic location, circular structure exon composition and back splicing site of circLRFN5. The back-splicing site of circLRFN5 was validated by Sanger sequencing. e, f Agarose gel electrophoresis showed the expression of circLRFN5 in cDNA and gDNA samples from GSC51 (e) and GSC53 (f) using divergent and convergent primers. β-actin served as a linear RNA control. g, h RNase treatment was used to evaluate the stability of circLRFN5 and LRFN5 mRNA in GSC51 (g) and GSC53 (h). i FISH assays showed the cellular localization of circLRFN5 in GSC51 and GSC53 cells. Scale bar = 200 μm. j The expression of circLRFN5 in 70 different WHO-grade glioma tissues as measured by qPCR. k The survival prognosis in different circLRFN5 expression groups was detected in 70 glioma patients. All data are expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001