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. 2022 Oct 20;41:307. doi: 10.1186/s13046-022-02518-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — CircLRFN5 inhibits GSCs viability, proliferation, neurospheres formation and stemness. a qPCR showed the expression of circLRFN5 in six GBM patients derived GSCs. b, c MTS assays showed the cell viabilities of GSC51 (b) and GSC58 (c) after circLRFN5 overexpression. d Representive EdU assays showed the proliferation of GSC51 and GSC58 after circLRFN5 overexpression. Scale bar = 50 μm. e Representive neurospheres formation assays showed the relative sizes of neurospheres after circLRFN5 overexpression in GSC51 and GSC58. Scale bar = 20 μm. f ELDA assays showed the neurospheres formation abilities of GSC51 (left) and GSC58 (right) after circLRFN5 overexpression. g Western blotting detects the stemness markers of GSCs (NANOG, OCT4, CD133 and SOX2) after circLRFN5 overexpression in GSC51 and GSC58. h Cell cycle assays showed the cell cycle distributions of GSC51 and GSC58 after circLRFN5 overexpression. All data are expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001