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. 2022 Oct 20;41:307. doi: 10.1186/s13046-022-02518-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — CircLRFN5 downregulates PRRX2 mediated transcription of GCH1 in GSCs. a A Venn Diagram showed the correlation between PRRX2 and ferroptosis-related genes in TCGA and CGGA datasets (R more than 0.4). b, c The correlation between PRRX2 and GCH1 in TCGA (b) and CGGA (c) datasets. d Sequence motif representing the consensus PRRX2 binding motif according to the JASPAR database. e Schematic diagram of the putative PRRX2 binding site in the promoter of GCH1. f–h Western blotting (f) and qPCR (g, h) showed the expression of GCH1 in circLRFN5 overexpressed GSC51 and GSC58, followed by PRRX2 overexpression. i, j The luciferase reporter assays showed the luciferase promoter activities of GCH1 after circLRFN5 overexpression in GSC51 (i) and GSC58 (j), followed by PRRX2 overexpression. k, l The ChIP qPCR showed that anti-PRRX2 treatment could lead to the enrichment of GCH1 in circLRFN5 overexpressed GSC51 (k) and GSC58 (l), followed by PRRX2 overexpression. All data are expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001