Figure 2.

Phylogenetic analysis and conserved motifs of Nramp proteins in stylo and other plants. The first two letters of each Nramp member indicate the plant species. At, Arabidopsis thaliana; Os, Oryza sativa; Mt, Medicago truncatula. The red arrows indicate SgNramps from stylo. The sequences of Nramp proteins were obtained from the previous studies (Tejada-Jiménez et al., 2015; Qin et al., 2017; Mani and Sankaranarayanan, 2018). The phylogenetic tree was constructed by MEGA4. Conserved motifs of Nramp proteins were analyzed by MEME. Each motif is presented by a colored box. The sequences of different motifs of Nramp proteins were summarized in Supplementary Table S3 .