Figure 5.

Genotypic variation analysis of SgNramp1. (A) Analysis of correlation between SgNramp1 expression and root Mn concentration in various stylo cultivars under 0.1 μM MnSO₄ treatments. The red and blue arrows indicate Reyan NO.18 and Reyan NO.22 cultivars, respectively. (B) Mn concentration in roots of Reyan NO.18 and Reyan NO.22 under 0.1 or 5 μM MnSO₄ treatments. (C) Expression of SgNramp1 in roots of Reyan NO.18 and Reyan NO.22 under 0.1 or 5 μM MnSO₄ treatments. Fourteen-day-old stylo seedlings were subjected to 0.1 or 5 μM MnSO₄ treatments for 7 days that were considered as low Mn and normal Mn treatments, respectively. Stylo roots were used for SgNramp1 expression analysis and Mn determination. Data are means of three biological replicates with SE. Different letters represent significant differences at P < 0.05.