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. 2022 Oct 6;13:1027551. doi: 10.3389/fpls.2022.1027551

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Copyright © 2022 Zou, Huang, Wang, Wang, Miao, Rao, Liu and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of SgNramp1. The 35:SgNramp1-GFP construct and 35:GFP empty vector were transiently expressed in tobacco epidermis cells. SgNramp1 was co-expressed with the plasma membrane (PM) marker, which was fused with red fluorescence protein (mKATE). Signals of GFP fusion protein, PM marker, chlorophyll autofluorescence (Auto), bright-field images (DIC), and the merged images (Merge) were shown from left to right. Scale bar is 20 μm.