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Manganese transport analysis of SgNramp1 in yeast. The yeast mutant Δsmf1 expressing either the pYES2 empty vector or SgNramp1 was spotted on SC-U/Gal solid medium containing 0, 20, or 25 mM Mn-chelator EGTA. The photo shows the growth of yeast cells at 30°C for 2 days.
