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. 2022 Oct 6;13:1027551. doi: 10.3389/fpls.2022.1027551

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Copyright © 2022 Zou, Huang, Wang, Wang, Miao, Rao, Liu and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth and Mn uptake in yeast mutant Δsmf1 expressing SgNramp1. (A) The yeast mutant Δsmf1 expressing either the pYES2 empty vector or SgNramp1 was grown in SC-U/Gal liquid medium with or without 20 mM EGTA for 0h to 48h. (B) Mn concentrations in the yeast cells grown in SC-U/Gal liquid medium containing 0, 20, or 40 μМ MnSO₄ for 24h. (C) The kinetics of Mn uptake in yeast cells. The kinetics parameters are shown next to the figure. Data are means of three biological replicates with SE. Asterisks indicate significant differences between the pYES2 empty vector and SgNramp1. **0.001 < P < 0.01; ***P < 0.001.