Figure 3.

Daphnia as a diagnostic early warning system. (A) Map of 30 water sampling sites along the Chaobai river basin, including three main streams (B, Bai river; C, Chao River; M, Chaobai river). These 30 water samples are subjected to targeted chemical analysis to identify 22 organic compounds (mostly pharmaceuticals) listed in Table S1. (B) Six putative mKEs identified via coexpression network analysis, are significantly enriched in xenobiotic and drug metabolic pathways identified in D. magna exposed to the Chaobai river water samples. The minus log-transformed adjusted P values are plotted in this figure; (C) Heatmap shows correlations between six putative mKEs and three pharmaceuticals: carbamazepine (CBZ), erythromycin (ERY), and trimethoprim (TMP). The color coding of the correlation coefficient increases from white (0) to dark purple (1), with asterisks (*) marking the significant correlations.