FIGURE 3.

CASZ1 increases proliferation and invasion abilities of glioma cells. (A and B) The relative expressions of CASZ1 were detected in U87‐MG and LN229 cell lines after transfection with overexpressed CASZ1 plasmids and siRNA by Western blot. (C and D) CCK8 assay was used to assess glioma cell proliferation after transfection with overexpressed CASZ1 plasmids and siRNA. (E and F) Colony‐formation assay was performed to determine the proliferation ability of U87 and LN229 cells infected with si‐NC and si‐CASZ1#1. (G) Transwell assay of U87‐MG and LN229 cell lines’ invasive ability in different groups. Scale bar = 100 μm. (H) Association between CASZ1 and Ki‐67/vimentin expression in clinical glioma specimens. Scale bar = 100 μm. (I) Correlation of CASZ1 expression with proliferation and invasion indices in 422 glioma patients