FIGURE 4.

CASZ1 promotes the proliferation and invasion ability of glioma cells by upregulating the transcription process of p75NTR gene. (A) GSEA enrichment plots showed that enrichment of cancer‐related pathways was associated with upregulation of CASZ1. (B) Volcano map shows all the differential genes between high and low CASZ1 groups. (C–F) CASZ1 gene positively regulates the mRNA and protein levels of p75NTR gene was evident by qRT‐PCR and Western blot assays in U87‐MG and LN229. (G–J) CCK8 and transwell assays were used to validate that CASZ1 regulated the proliferation and invasion abilities of glioma cells through p75NTR. Scale bar = 100 μm. (K) ChIP‐qRT‐PCR assay performed using anti‐CASZ1 antibody. Rabbit IgG was used as a negative immunoprecipitation control. (L) Luciferase reporter assay of p75NTR transcriptional activity. (M) CASZ1 transcription factor increased p75NTR promoter activity by luciferase reporter assay. TSS: transcription start site